Reaction of Prostaglandin Endoperoxide Synthase with cis,cis-Eicosa-11,14-dienoic Acid
نویسندگان
چکیده
منابع مشابه
Reaction of prostaglandin endoperoxide synthase with cis,cis-eicosa-11,14-dienoic acid.
The pre-steady-state phase of the oxygenase reaction of prostaglandin endoperoxide synthase with cis,cis-eicosa-11, 14-dienoic acid has been studied using stopped flow techniques. Because some intermediate forms of prostaglandin endoperoxide synthase are spectrally indistinguishable, the enzyme and substrate transformations were monitored in parallel to simplify the interpretation of the kineti...
متن کاملAdvances on prostaglandin - endoperoxide synthase 2 gene
Prostaglandin-endoperoxide synthase 2 is the rate-limiting enzyme in the biosynthesis of prostaglandins (PGs). In this paper, we briefly introduced the structure, expression, regulation mechanism of prostaglandinendoperoxide synthase 2 gene and the relationship between this gene and reproductive performance.
متن کاملProstaglandin endoperoxide synthase: why two isoforms?
Prostaglandin endoperoxide synthase-1 [prostaglandin G/H synthase-1 (PGHS-1)] and PGHS-2 are key enzymes in the conversion of arachidonic acid to prostaglandins and other eicosanoids. We refer to these isoforms as cyclooxygenase-1 (COX-1) and COX-2 in this review. This brief review focuses on recent developments in the study of these enzymes. Alterations in the expression levels of COX-2 result...
متن کاملUnique peroxidase reaction mechanism in prostaglandin endoperoxide H synthase-2: compound I in prostaglandin endoperoxide H synthase-2 can be formed without assistance by distal glutamine residue.
Prostaglandin-endoperoxide H synthase-2 (PGHS-2) shows peroxidase activity to promote the cyclooxygenase reaction for prostaglandin H2, but one of the highly conserved amino acid residues in peroxidases, distal Arg, stabilizing the developing negative charge on the peroxide through a hydrogen-bonding interaction, is replaced with a neutral amino acid residue, Gln. To characterize the peroxidase...
متن کاملPTGS 2 ( prostaglandin - endoperoxide synthase 2 ( prostaglandin G / H synthase and cyclooxygenase ) )
COX2 is an enzyme that belongs to the prostaglandin G/H synthase family. It consists of 604 amino acids and has a molecular weight of 68996 Da. COX2 possesses two catalytic activities and respective active sites: a cyclooxygenase (COX) that converts arachidonic acid to a prostaglandin endoperoxide, prostaglandin G2 (PGG2), and; a peroxidase (POX) that reduces PGG2 to PGH2. COX2 functions as hom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.11.6046